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17:00 - 18:00 6 October 2011

Monitoring neuronal signalling in 3D space with millisecond resolution using an Acousto-Optic Lens two-photon Microscope (AOLM)


4th Floor Seminar Room | Wellcome Trust Centre for Neuroimaging (link Map)
12 Queen Square | London | WC1N 3BG | United Kingdom

Open to: Academic | Student

Speaker information

Dr Tomas Fernandez Alfonso, NPP, UCL

Speaker: Dr Tomas Fernandez Alfonso, Dept. of Neuroscience, Physiology & Pharmacology, UCL. Host: Centre for Neuroimaging Techniques. Abstract: Two photon (2P) microscopy is widely used to study brain function at the synaptic, neuronal and network level, however current galvanometer-based microscopes typically take > 100ms to form a 2D image and mechanical focusing is slow. I will describe an Acousto Optic Lens Microscope (AOLM) for 2P functional imaging at high speed in 3D and show our first biological results testing its utility by measuring Action Potential (AP)-evoked Ca2+ transients both in vitro and in vivo. I will present results both in the dendritic tree of a single neuron and also in a population of neurons in the visual cortex of anaesthetized mouse that demonstrate that the AOLM has the speed and sensitivity to detect rapid neuronal signalling in 3D.


Louis Lemieux
Please email | louis.lemieux@ucl.ac.uk


Centre for Neuroimaging Techniques